 | ARR GP40-2HEP | Alaska Railroad | | | 2 |
| B&O GP40-2 | Baltimore and Ohio Railroad | | | 3 |
| BN GP40-2 | Burlington Northern | | | 2 |
| BNSF GP40-2 | BNSF Railway | | | 3 |
| C&O GP40-2 | Chesapeake and Ohio Railway | | | 2 |
 | CN GP40-2LW | Canadian National Railway | | | 4 |
| CORP GP40-2 | Central Oregon and Pacific Railroad | | | 0 |
| CR GP40-2 | Conrail: Consolidated Rail Corporation | | | 5 |
| CS GP40-2 | Chessie System, Inc. | | | 1 |
| CSXT GP38-2S | CSX Transportation | | | 1 |
| CSXT GP40-2 | CSX Transportation | | | 10 |
| CXRG GP40-2 | Colorado Pacific Rio Grande | | | 1 |
| DRGW GP40-2 | Denver & Rio Grande Western Railroad | | | 4 |
| DTI GP40-2 | Detroit Toledo & Ironton Railway | | | 2 |
| ECRX GP40-2 | Trans-Global Solutions, Inc. | | | 1 |
| FEC GP40-2 | Florida East Coast Railway | | | 15 |
| GTW GP40-2 | Grand Trunk Western Railroad | | | 1 |
| GWR GP40-2LW | Great Western Railway of Colorado | | | 1 |
| HESR GP40-2 | Huron and Eastern Railway | | | 1 |
| IANR GP40-2LW | Iowa Northern Railway | | | 2 |
| ICE GP40-2 | Iowa, Chicago and Eastern Railroad | | | 1 |
| IHB GP40-2 | Indiana Harbor Belt Railroad | | | 2 |
| KCS GP40-2 | Kansas City Southern Railway | | | 1 |
| NJT GP40-2 | New Jersey Transit | | | 3 |
| NS GP40-2 | Norfolk Southern Railway | | | 2 |
| PRLX GP40-2 | Progress Rail | | | 1 |
| QGRY GP40-2 | Quebec Gatineau Railway | | | 5 |
| RMRX GP40-2LW | Rocky Mountaineer | | | 2 |
| RRRR GP40-2 | Rock & Rail | | | 1 |
| SCL GP40-2 | Seaboard Coast Line Railroad | | | 1 |
| SLSF GP40-2 | St. Louis & San Francisco Railway (Frisco) | | | 1 |
| SOO GP40-2 | Soo Line Railroad | | | 1 |
| SP GP40-2 | Southern Pacific Transportation Company | | | 2 |
| SP GP40M-2 | Southern Pacific Transportation Company | | | 3 |
| SSW GP40-2 | St. Louis Southwestern Railway | The Cotton Belt Route | | 3 |
| UP GP40-2 | Union Pacific Railroad | | | 4 |
| UP GP40M-2 | Union Pacific Railroad | | | 2 |
| UP GP40N | Union Pacific Railroad | | | 3 |
| USSC GP40-2 | United States Sugar Corporation | | | 7 |
| VTR GP40-2 | Vermont Railway | | | 2 |
| VTR GP40-2W | Vermont Railway | | | 1 |
| WAMX GP40-2 | Webb Asset Management | | | 1 |
| WP GP40-2 | Western Pacific Railroad | | | 3 |
