 | ATSF GP35 | Atchison, Topeka and Santa Fe Railway | | | 2 |
| B&O GP35 | Baltimore and Ohio Railroad | | | 1 |
| BN GP35 | Burlington Northern | | | 7 |
| BNSF GP35U | BNSF Railway | | | 1 |
| BNSF GP39E | BNSF Railway | | | 7 |
| BNSF GP39V | BNSF Railway | | | 1 |
| CNW GP35 | Chicago and North Western Transportation Company | | | 1 |
| DRGW GP35 | Denver & Rio Grande Western Railroad | | | 1 |
| GLC GP35 | Great Lakes Central Railroad | | | 1 |
| GM&O GP35 | Gulf Mobile & Ohio | | | 1 |
| HRRC GP35 | Housatonic Railroad | | | 3 |
| ICG GP35 | Illinois Central Gulf | | | 1 |
| KFR GP35 | Kettle Falls International Railway | | | 1 |
| LVRR GP35 | Lycoming Valley Railroad | | | 1 |
| MP GP35 | Missouri Pacific | | | 2 |
| MRL GP35 | Montana Rail Link | | | 3 |
| NW GP35 | Norfolk and Western Railway | | | 1 |
| RLK GP35M | RailAmerica | | | 1 |
| SLSF GP35 | St. Louis & San Francisco Railway (Frisco) | | | 1 |
| SMV GP35 | Santa Maria Valley Railroad | | | 1 |
| SOU GP35 | Southern Railway | | | 1 |
| SP GP35 | Southern Pacific Transportation Company | | | 1 |
| SP GP35R | Southern Pacific Transportation Company | | | 1 |
| TSBY GP35 | Tuscola and Saginaw Bay Railway | | | 1 |
| UP GP35 | Union Pacific Railroad | | | 6 |
| WAMX GP35R | Webb Asset Management | | | 3 |
| WC GP35 | Wisconsin Central Ltd. | | | 1 |
| WE GP35 | Wheeling and Lake Erie Railway | | | 3 |
| WE GP35-3 | Wheeling and Lake Erie Railway | | | 1 |
| WVR GP35 | Willamette Valley Railway | | | 2 |
